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- Title
A promiscuous α-helical motif anchors viral hijackers and substrate receptors to the CUL4–DDB1 ubiquitin ligase machinery.
- Authors
Ti Li; Robert, Eva I.; van Breugel, Pieter C.; Strubin, Michel; Ning Zheng
- Abstract
The cullin 4–DNA-damage-binding protein 1 (CUL4–DDB1) ubiquitin ligase machinery regulates diverse cellular functions and can be subverted by pathogenic viruses. Here we report the crystal structure of DDB1 in complex with a central fragment of hepatitis B virus X protein (HBx), whose DDB1-binding activity is important for viral infection. The structure reveals that HBx binds DDB1 through an α-helical motif, which is also found in the unrelated paramyxovirus SV5-V protein despite their sequence divergence. Our structure-based functional analysis suggests that, like SV5-V, HBx captures DDB1 to redirect the ubiquitin ligase activity of the CUL4–DDB1 E3 ligase. We also identify the α-helical motif shared by these viral proteins in the cellular substrate–recruiting subunits of the E3 complex, the DDB1–CUL4-associated factors (DCAFs) that are functionally mimicked by the viral hijackers. Together, our studies reveal a common yet promiscuous structural element that is important for the assembly of cellular and virally hijacked CUL4–DDB1 E3 complexes.
- Subjects
DNA-binding proteins; UBIQUITIN; HEPATITIS B virus; PARAMYXOVIRUSES; VIRAL proteins
- Publication
Nature Structural & Molecular Biology, 2010, Vol 17, Issue 1, p105
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.1719