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- Title
Regulated degradation of replication-dependent histone mRNAs requires both ATR and Upf1.
- Authors
Kaygun, Handan; Marzluff, William F
- Abstract
Eukaryotic cells coordinately regulate histone and DNA synthesis. In mammalian cells, most of the regulation of histone synthesis occurs post-transcriptionally by regulating the concentrations of histone mRNA. As cells enter S phase, histone mRNA levels increase, and at the end of S phase they are rapidly degraded. Moreover, inhibition of DNA synthesis causes rapid degradation of histone mRNAs. Replication-dependent histone mRNAs are the only metazoan mRNAs that are not polyadenylated. Instead, they end with a conserved stem-loop structure, which is the only cis-acting element required for coupling regulation of histone mRNA half-life with DNA synthesis. Here we show that regulated degradation of histone mRNAs requires Upf1, a key regulator of the nonsense-mediated decay pathway, and ATR, a key regulator of the DNA damage checkpoint pathway activated during replication stress.
- Subjects
MESSENGER RNA; HISTONES; BASIC proteins; CHROMATIN; NUCLEOPROTEINS; RNA
- Publication
Nature Structural & Molecular Biology, 2005, Vol 12, Issue 9, p794
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb972