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- Title
Quantitative Stereology:The Use of Camera Lucida for Counting Neurons by Physical Disector Method in Chick Brainstem Auditory Nuclei.
- Authors
Wadhwa, S.
- Abstract
The article focuses on the disector introduced in 1984 by scientist D.C. Sterio that is an unbiased, systematically randomized method for neuronal counting applied as an optical or physical method. The optical disector requires a confocal microscope or a conventional light microscope with high precision control and calibration of movement in depth through the 'z' axis. The physical disector requires overlapping of adjacent sections to eliminate overestimation in counting the neurons as may occur with the use of single sections. The article further reports the experience of using the camera lucida over the last ten years in counting the neurons in chick brainstem auditory nuclei.
- Subjects
CONFOCAL microscopy; STERIO, D. C.; CAMERA lucidas; NEUROPLASTICITY; NEURONS -- Ultrastructure; CALIBRATION
- Publication
Journal of Postgraduate Medicine, 2003, Vol 49, Issue 4, p376
- ISSN
0022-3859
- Publication type
Article