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- Title
Photosynthetic 1,8-cineole production using cyanobacteria.
- Authors
Yutaka Sakamaki; Mizuki Ono; Nozomi Shigenari; Taku Chibazakura; Kenji Shimomura; Satoru Watanabe
- Abstract
Terpenoid is an important group of compounds not only as biocomponents but also as useful secondary metabolites. A volatile terpenoid 1,8-cineole, which is used as a food additive, flavoring agent, cosmetic, etc., is also attracting attention from a medical perspective due to its antiinflammation and antioxidation. The 1,8-cineole fermentation using a recombinant Escherichia coli strain has been reported, although a carbon source supplement is necessary for a high-yield 1,8-cineole production. We constructed the 1,8-cineole-producing cyanobacteria toward a carbon-free and sustainable 1,8-cineole production. cnsA, the 1,8-cineole synthase gene in Streptomyces clavuligerus ATCC 27064, was introduced and overexpressed in the cyanobacterium Synechococcus elongatus PCC 7942. We succeeded in producing an average of 105.6 μg g −1 wet cell weight of 1,8-cineole in S . elongatus 7942 without supplementing any carbon source. Using the cyanobacteria expression system is an efficient approach to producing 1,8-cineole by photosynthesis.
- Subjects
CYANOBACTERIA; SUSTAINABILITY; SYNECHOCOCCUS elongatus; FOOD additives; METABOLITES; PHOTOSYNTHETIC bacteria
- Publication
Bioscience, Biotechnology & Biochemistry, 2023, Vol 87, Issue 5, p563
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbad012