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- Title
RNA-sequencing tag profiling of the placenta and pericarp of pungent pepper provides robust candidates contributing to capsaicinoid biosynthesis.
- Authors
Liu, Shaoqun; Chen, Changming; Chen, Guoju; Cao, Bihao; Chen, Qinghua; Lei, Jianjun
- Abstract
Capsaicinoids, metabolites produced in plant placental (seed-bearing) tissue, are responsible for the pungent taste associated with fruits of the genus Capsicum. To identify genes involved in the biosynthesis of capsaicinoids, genome-wide transcriptional profiling of the placenta and pericarp of pungent pepper ( C. frutescens L.) was performed using the RNA sequencing (RNA-Seq) approach. The results revealed 4,092 genes with significantly different expression levels between the placenta and pericarp libraries. Among them, 2,049 genes were up-regulated and 2,043 genes were down-regulated in the placenta compared to those of the pericarp. Hundreds of genes potentially regulating capsaicinoid were identified from the up-regulated genes, including genes previously associated with this metabolic process. Subsequent analysis of expression patterns for a subset of these identified genes were verified by semi-quantitative RT-PCR and quantitative RT-PCR. Furthermore, The differential expressed genes were predicted to be involved in microbody, peroxisome, fatty-acid synthase activity, CoA-ligase activity, acyltransferase activity, transaminase activity, phenylalanine metabolism, and other processes through gene ontology and KEGG enrichment analysis. Together, this study provides novel insights into the molecular mechanisms of capsaicinoid biosynthesis.
- Subjects
NUCLEOTIDE sequence; PLACENTA; PERICARP; PUNGENCY; BIOSYNTHESIS
- Publication
Plant Cell, Tissue & Organ Culture, 2012, Vol 110, Issue 1, p111
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-012-0135-8