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- Title
Increased polysaccharide production and biosynthetic gene expressions in a submerged culture of Ganoderma lucidum by the overexpression of the homologous α-phosphoglucomutase gene.
- Authors
Xu, Jun-Wei; Ji, Sen-Lin; Li, Huan-Jun; Zhou, Jiang-Sheng; Duan, Yan-Qing; Dang, Li-Zhi; Mo, Ming-He
- Abstract
This study aimed to improve the production of polysaccharide by engineering the biosynthetic pathway in Ganoderma lucidum through the overexpression of α-phosphoglucomutase (PGM) gene. PGM is responsible for the linkage between sugar catabolism and sugar anabolism. The effects of PGM gene overexpression on intracellular polysaccharide (IPS) content, extracellular polysaccharide (EPS) production and transcription levels of three genes encoding the enzymes involved in polysaccharide biosynthesis, including PGM, UDP-glucose pyrophosphorylase (UGP), and β-1,3-glucan synthase (GLS), were investigated. The maximum IPS content and EPS production in G. lucidum overexpressing the PGM gene were 23.67 mg/100 mg dry weight and 1.76 g/L, respectively, which were higher by 40.5 and 44.3 % than those of the wild-type strain. The transcription levels of PGM, UGP and GLS were upregulated by 4.77-, 1.51- and 1.53-fold, respectively, in the engineered strain, suggesting that increased polysaccharide biosynthesis may result from a higher expression of those genes.
- Subjects
POLYSACCHARIDE synthesis; GANODERMA lucidum; FUNGAL gene expression; MICROBIAL genetic engineering; FUNGAL cultures; EXTRACELLULAR enzymes; PHOSPHOGLUCOMUTASE; GENETIC overexpression
- Publication
Bioprocess & Biosystems Engineering, 2015, Vol 38, Issue 2, p399
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-014-1279-1