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- Title
An optrode array for spatiotemporally-precise large-scale optogenetic stimulation of deep cortical layers in non-human primates.
- Authors
Clark, Andrew M.; Ingold, Alexander; Reiche, Christopher F.; Cundy III, Donald; Balsor, Justin L.; Federer, Frederick; McAlinden, Niall; Cheng, Yunzhou; Rolston, John D.; Rieth, Loren; Dawson, Martin D.; Mathieson, Keith; Blair, Steve; Angelucci, Alessandra
- Abstract
Optogenetics has transformed studies of neural circuit function, but remains challenging to apply to non-human primates (NHPs). A major challenge is delivering intense, spatiotemporally-precise, patterned photostimulation across large volumes in deep tissue. Such stimulation is critical, for example, to modulate selectively deep-layer corticocortical feedback circuits. To address this need, we have developed the Utah Optrode Array (UOA), a 10×10 glass needle waveguide array fabricated atop a novel opaque optical interposer, and bonded to an electrically addressable µLED array. In vivo experiments with the UOA demonstrated large-scale, spatiotemporally precise, activation of deep circuits in NHP cortex. Specifically, the UOA permitted both focal (confined to single layers/columns), and widespread (multiple layers/columns) optogenetic activation of deep layer neurons, as assessed with multi-channel laminar electrode arrays, simply by varying the number of activated µLEDs and/or the irradiance. Thus, the UOA represents a powerful optoelectronic device for targeted manipulation of deep-layer circuits in NHP models. A novel device for selective large-scale optogenetic manipulation of the deep layers of cortical circuits in non-human primates is presented and validated using electrophysiological recordings and c-fos imaging in macaque visual cortex.
- Subjects
PRIMATES; NEURAL circuitry; OPTOELECTRONIC devices; VISUAL cortex; OPTOGENETICS; SUBTHALAMIC nucleus; MACAQUES
- Publication
Communications Biology, 2024, Vol 7, Issue 1, p1
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-024-05984-2