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- Title
Association of Antigen-Stimulated Release of Tumor Necrosis Factor-Alpha in Whole Blood with Response to Chemotherapy in Patients with Pulmonary Multidrug-Resistant Tuberculosis.
- Authors
Eum, Seok-Yong; Lee, Ye-Jin; Min, Jin-Hong; Kwak, Hyun-Kyung; Hong, Min-Sun; Kong, Ji-Hye; Hwang, Soo-Hee; Park, Seung-Kyu; LeBlanc, Jason J.; Via, Laura E.; Barry, III, Clifton E.; Cho, Sang-Nae
- Abstract
Background: We have previously reported that TNF-α levels correlate to total mycobacterial burden in tuberculosis (TB) patients. Objective: To characterize the dynamics of cytokine responses in TB patients during chemotherapy to identify potential surrogate markers for effective treatment. Methods: Following induction by culture filtrate proteins in whole blood, production patterns of TNF-α, IL-10, IFN-γ and IL-12 were measured in 23 non-multidrug-resistant (MDR)-TB and 16 MDR-TB patients and in 31 healthy controls. Rates of mycobacterial clearance from the sputum were then measured and compared. Results: Prior to the initiation of chemotherapy, TNF-α and IL-10 levels were significantly higher in TB patients than in healthy controls while IFN-γ and IL-12 levels were similar. During chemotherapy, the levels of all 4 cytokines increased. We evaluated these responses separately in patients that did and did not clear their sputum culture at 2 and 6 months. At 2 months, decreases in both IFN-γ and IL-12 correlated strongly with a successful early response, while after 6 months of therapy, when half (7/14) of MDR-TB patients were still sputum culture positive, downregulation of TNF-α was uniquely correlated with sputum conversion between the groups. Conclusion: Our findings suggest the possibility that the regulation of TNF-α production in whole blood may be a more specific indicator of sputum conversion at 6 months than IFN-γ, IL-12 or IL-10 in MDR-TB patients. Copyright © 2010 S. Karger AG, Basel
- Subjects
KOREA; ANTITUBERCULAR agents; BIOMARKERS; COMPARATIVE studies; CYTOKINES; DRUG monitoring; ENZYME-linked immunosorbent assay; HOSPITALS; INTERFERONS; INTERLEUKINS; LONGITUDINAL method; RESEARCH funding; SPUTUM; TUMOR necrosis factors; U-statistics; MULTIDRUG-resistant tuberculosis; BLOOD; ANALYTICAL chemistry; PHARMACODYNAMICS; IMMUNOLOGY; DRUG therapy
- Publication
Respiration, 2010, Vol 80, Issue 4, p275
- ISSN
0025-7931
- Publication type
Article
- DOI
10.1159/000283687