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- Title
Isolation of a methyl parathion-degrading strain Stenotrophomonas sp. SMSP-1 and cloning of the ophc2 gene.
- Authors
Yu-jia Shen; Pen Lu; Huan Mei; Hao-jie Yu; Qing Hong; Shun-peng Li
- Abstract
A rod-shaped, gram-negative bacterium Stenotrophomonas sp. SMSP-1 was isolated from the sludge of a wastewater treating system of a pesticide manufacturer. Strain SMSP-1 could hydrolyze methyl parathion to p-nitrophenol (PNP) and dimethyl phosphorothioate but could not degrade PNP further. Strain SMSP-1 was able to hydrolyze other organophosphate pesticides, including fenitrothion, ethyl parathion, fenthion, and phoxim, but not chlorpyrifos. A 4395-bp DNA fragment, including an organophosphorus hydrolase encoding gene ophc2, was cloned from the chromosome of strain SMSP-1 using the shotgun technique. Its sequence analysis showed that ophc2 was associated with a typical mobile element IS Ppu12 consisting of tnpA (encoding a transposase), lspA (encoding a lipoprotein signal peptidase), and orf1 (encoding a CDF family heavy metal/H+ antiporter). The ophc2 gene was effectively expressed in E. coli. This is the second report of cloning the ophc2 gene and the first report of this gene from the genus of Stenotrophomonas.
- Subjects
PARATHION; METHYL groups; WASTE management; PHOSPHAMIDON; CHOLINESTERASE reactivators; CHLORPYRIFOS; BIODEGRADATION; AGRICULTURAL ecology; AGRICULTURAL chemicals
- Publication
Biodegradation, 2010, Vol 21, Issue 5, p785
- ISSN
0923-9820
- Publication type
Article
- DOI
10.1007/s10532-010-9343-2