We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Comparative evaluation of in vivo biocompatibility and biodegradability of regenerated silk scaffolds reinforced with/without natural silk fibers.
- Authors
Mobini, Sahba; Taghizadeh-Jahed, Masoud; Khanmohammadi, Manijeh; Moshiri, Ali; Naderi, Mohammad-Mehdi; Heidari-Vala, Hamed; Ashrafi Helan, Javad; Khanjani, Sayeh; Springer, Armin; Akhondi, Mohammad-Mehdi; Kazemnejad, Somaieh
- Abstract
Nowadays, exceptional advantages of silk fibroin over synthetic and natural polymers have impelled the scientists to application of this biomaterial for tissue engineering purposes. Recently, we showed that embedding natural degummed silk fibers in regenerated Bombyx mori silk-based scaffold significantly increases the mechanical stiffness, while the porosity of the scaffolds remains the same. In the present study, we evaluated degradation rate, biocompatibility and regenerative properties of the regenerated 2% and 4% wt silk-based composite scaffolds with or without embedded natural degummed silk fibers within 90 days in both athymic nude and wild-type C57BL/6 mice through subcutaneous implantation. In all scaffolds, a suitable interconnected porous structure for cell penetration was seen under scanning electron microscopy. Compressive tests revealed a functional relationship between fiber reinforcement and compressive modulus. In addition, the fiber/fibroin composite scaffolds support cell attachment and proliferation. On days 30 to 90 after subcutaneous implantation, the retrieved tissues were examined via gross morphology, histopathology, immunofluorescence staining and reverse transcription-polymerase chain reaction as shown in Figure 1. Results showed that embedding the silk fibers within the matrix enhances the biodegradability of the matrix resulting in replacement of the composite scaffolds with the fresh connective tissue. Fortification of the composites with degummed fibers not only regulates the degradation profile but also increases the mechanical performance of the scaffolds. This report also confirmed that pore size and structure play an important role in the degradation rate. In conclusion, the findings of the present study narrate key role of additional surface area in improving in vitro and in vivo biological properties of the scaffolds and suggest the potential ability of these fabricated composite scaffolds for connective tissue regeneration. <named-content> Illustrative summary of the main methods and findings. RS: regenerated silk; RSF: regenerated fibroin/ silk fiber composite scaffolds; H&E: Hematoxylin and eosin; COX-1: Cyclooxygenase.</named-content><graphic></graphic>
- Subjects
BIOCOMPATIBILITY; BIODEGRADATION; SCANNING electron microscopy; COMPARATIVE studies; TISSUE engineering
- Publication
Journal of Biomaterials Applications, 2016, Vol 30, Issue 6, p793
- ISSN
0885-3282
- Publication type
Article
- DOI
10.1177/0885328215601925