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- Title
DIAGNOSTIC UTILITY OF THE HEP PAR-1 TO DIFFERENTIATE HEPATOCELLULAR CARCINOMA FROM METASTATIC CARCINOMA ON CONVENTIONAL TISSUE SECTIONS.
- Authors
Basa, Norina; Lazăr, Elena; Cornianu, Marioara; Dema, Alis; Tăban, Sorina; Lazăr, Daniela; Ancusa, D.; Mureşan, Anca; Grigoraş, Mirela; Cornea, R.; Popescu, Roxana
- Abstract
Hepatocellular carcinoma (HCC) is the most common primary liver neoplasm, its differentiation from other malignant tumors being sometimes difficult without a reliable immunohistochemical marker for hepatocellular differentiation. Hep Par-1 (Hepatocyte paraffin-1), an antibody specific for hepatocytes, reacts with normal and neoplastic hepatocytes. We assessed the expression of Hep Par-1 on tissue sections included in paraffin, from patients clinically diagnosed with liver tumors submitted to curative resection (22 cases), on liver tumor fragments obtained by laparoscopy (six cases) and others obtained at necropsy (five cases). We used the anti-human hepatocyte mouse monoclonal antibody, Hepatocyte Ab- 1, clone OCH1E5, diluted 1:40, using the EnVision technique. We obtained: 1) positive Hep Par-1 expression in 18 of the 21 cases of HCC (85.71%), dependent of the differentiation degree (positive in all 16 G1-G2 HCCs and negative in 5 G3-G4 HCCs); 2) heterogenic or patchy Hep Par-1 distribution in tumor tissue and a uniform staining of the non-neoplastic liver surrounding the tumor. We did not note Hep Par-1 immunostaining in non-hepatocytic tumors, except one case of gastric carcinoma and cholangiocarcinoma (CC) with focal expression. Hep Par-1 is a useful marker in the differential diagnosis of HCC, but not entirely specific; its usage in association with other markers for hepatocellular differentiation can help in the correct diagnosis of HCC and its differentiation from CC and metastatic liver tumors.
- Subjects
LIVER cancer; IMMUNOHISTOCHEMISTRY; LIVER cell differentiation; IMMUNOGLOBULINS; CHOLANGIOCARCINOMA
- Publication
Annals of the Romanian Society for Cell Biology, 2011, Vol 16, Issue 1, p261
- ISSN
2067-3019
- Publication type
Article