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- Title
作物基因编辑效果可视化鉴定软件.
- Authors
尤琪; 吴文文; 姜艺
- Abstract
The CRISPR editing system together with high-throughput sequencing can evaluate the efficiency and accuracy of mutation efficiently. At present, they have been widely used in genetics, biology, medicine and others. However, a one-click visualization tool was lack to evaluate mutation efficiency and accuracy of high-throughput sequencing data. Here, a Python-based visualization tool was developed, named as the visualization software for plant gene editing identification. The software can calculate high-throughput gene editing data together, and can realize data preprocessing (split and merge), detection of mutation and efficiency, visualization outputs of charts with one click. The software was an interface program, data input and results output could be realized by clicking the mouse. The outputs had a variety of formats, including images zooming and color change, which was convenient for post-processing results and writing papers. In addition, the tool supported multiple operating systems (Windows, Linux and MacOS) and was easy to install and use. At present, the user manual and program package of the software had been uploaded to the open software platform.
- Subjects
GENOME editing; DATA editing; NUCLEOTIDE sequencing; PLANT genes; INTEGRATED software; PYTHON programming language; LINUX operating systems
- Publication
Acta Agriculturae Zhejiangensis, 2022, Vol 34, Issue 12, p2759
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2022.12.19