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- Title
High-voltage electron microscopy tomography and structome analysis of unique spiral bacteria from the deep sea.
- Authors
Masashi Yamaguchi; Hiroyuki Yamada; Kimitaka Higuchi; Yuta Yamamoto; Shigeo Arai; Kazuyoshi Murata; Yuko Mori; Hiromitsu Furukawa; Uddin, Mohammad Shorif; Hiroji Chibana
- Abstract
Structome analysis is a useful tool for identification of unknown microorganisms that cannot be cultured. In 2012, we discovered a unique deep-sea microorganism with a cell structure intermediate between those of prokaryotes and eukaryotes and described its features using freeze-substitution electron microscopy and structome analysis (quantitative and three-dimensional structural analysis of a whole cell at the electron microscopic level). We named it Myojin parakaryote. Here we describe, using serial ultrathin sectioning and high-voltage electron microscopy tomography of freeze-substituted specimens, the structome analysis and 3D reconstruction of another unique spiral bacteria, found in the deep sea off the coast of Japan. The bacteria, which is named as 'Myojin spiral bacteria' after the discovery location and their morphology, had a total length of 1.768 ± 0.478 μm and a total diameter of 0.445 ± 0.050 μm, and showed either clockwise or counter-clockwise spiral. The cells had a cell surface membrane, thick fibrous layer, ribosomes and inner fibrous structures (most likely DNA). They had no flagella. The bacteria had 322 ± 119 ribosomes per cell. This ribosome number is only 1.2% of that of Escherichia coli and 19.3% of Mycobacterium tuberculosis and may reflect a very slow growth rate of this organismin the deep sea.
- Subjects
ELECTRON microscopy; MARINE bacteria; TOMOGRAPHY; MARINE microorganisms; PROKARYOTES; EUKARYOTES
- Publication
Microscopy, 2016, Vol 65, Issue 4, p363
- ISSN
2050-5698
- Publication type
Article
- DOI
10.1093/jmicro/dfw016