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- Title
Immune response of largemouth bass, Micropterus salmoides, to whole cells of different Nocardia seriolae strains.
- Authors
Shimahara, Yoshiko; Yun-Fen Huang; Ming-An Tsai; Pei-Chi Wang; Shih-Chu Chen
- Abstract
Largemouth bass Micropterus salmoides were immunized with four different N. seriolae strains—two α-glucosidase-positive (961113, KU040801) and two α-glucosidase-negative (94260, OTTS) strains—along with Freund’s incomplete adjuvant. After primary immunization (week 0), a booster was administered at weeks 4 and 8. Nonspecific immune responses to multiple immunizations with the different N. seriolae strains were determined based on serum lysozyme activity and nitroblue tetrazolium (NBT)-positive cells in peripheral blood. The serum lysozyme activity and NBT-positive cells in peripheral blood were not significantly increased even after the two booster immunizations. Specific antibody responses against N. seriolae cells were investigated by enzyme-linked immunosorbent assay. At 4 weeks after immunization, all groups immunized with N. seriolae antigens showed significant increases in their specific antibody levels. The sera from fish immunized with different N. seriolae strains exhibited reactivity with N. seriolae sonicated antigens of 28, 30, 36 and 84 kDa by western blot analysis. After two boosters, fish were challenged with live N. seriolae to assess the vaccine’s efficacy; however, multiple injections of the N. seriolae strains did not reduce mortality, irrespective of the bacterin.
- Subjects
LARGEMOUTH bass; IMMUNIZATION; ANTIGENS; IMMUNOGLOBULINS; BLOOD; LYSOZYMES
- Publication
Fisheries Science, 2010, Vol 76, Issue 3, p489
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1007/s12562-010-0239-1