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- Title
AMY-1, a novel C-MYC binding protein that stimulates transcription activity of C-MYC.
- Authors
Taira, Takahiro; Maëda, Junko; Onishi, Takako; Kitaura, Hirotake; Yoshida, Shu; Kato, Hiroyuki; Ikeda, Masako; Tamai, Katsuyuki; M. Iguchi-Ariga, Sanae M.; Ariga, Hiroyoshi
- Abstract
BackgroundThe c-myc proto-oncogene has been suggested to play key roles in cell proliferation, differentiation, transformation and apoptosis. A variety of functions of C-MYC, the product of c-myc, are attributed to protein–protein interactions with various cellular factors including Max, YY1, p107, Bin1 and TBP. Max and YY1 bind to the C-terminal region of C-MYC, while p107, Bin1 and TBP bind to the N-terminal region covering myc boxes. The N-terminal region is involved in all the biological functions of C-MYC, and different proteins are therefore thought to interact with the N-terminal region of C-MYC to display different functions. ResultsWe cloned two cDNAs which encode a novel C-MYC-binding protein of 11 kDa, designated AMY-1 (Associate of C-MYC). The two cDNAs, AMY-1L and AMY-1S, derived from alternative usage of polyadenylation signals, code for the same protein of 11 kDa. AMY-1 was bound via its C-terminal region to the N-terminal region of C-MYC (amino acids nos 58–148) corresponding to the transactivation domain. AMY-1 was localized in the cytoplasm in cells expressing c-myc at low levels, but in the nucleus in the cells of a high c-myc expression in transiently transfected cells. A similar difference in endogenous AMY-1 localization was observed during the cell cycle: AMY-1 translocated from cytoplasm to nucleus during the S phase when c-myc expression was increased. AMY-1 by itself did not recognize the E-box element, the MYC/Max binding sequence, nor did it transactivate via the element, but stimulated the activation of E-box-regulated transcription by MYC/Max. FISH analyses revealed that the amy-1 gene was located at 1p32.2–1p33 in human genome. ConclusionsAMY-1 is a 11 kDa protein which binds to the N-terminal region of C-MYC and stimulates the activation of E-box-dependent transcription by C-MYC. AMY-1, which mostly localizes in the cytoplasm, translocates into the nucleus in the S phase of the cell cycle upon an increase of...
- Subjects
CARRIER proteins; GENETIC transcription; ONCOGENES
- Publication
Genes to Cells, 1998, Vol 3, Issue 8, p549
- ISSN
1356-9597
- Publication type
Article
- DOI
10.1046/j.1365-2443.1998.00206.x