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- Title
Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole–imidazole polyamide, which targets an E-box motif.
- Authors
Taniguchi, Masashi; Fujiwara, Kyoko; Nakai, Yuji; Ozaki, Toshinori; Koshikawa, Nobuko; Toshio, Kojima; Kataba, Motoaki; Oguni, Asako; Matsuda, Hiroyuki; Yoshida, Yukihiro; Tokuhashi, Yasuaki; Fukuda, Noboru; Ueno, Takahiro; Soma, Masayoshi; Nagase, Hiroki
- Abstract
Gene amplification and/or overexpression of the transcription factor c-MYC, which binds to the E-box sequence (5′-CACGTG-3′), has been observed in many human tumors. In this study, we have designed 5 pyrrole–imidazole (PI) polyamides recognizing E-box, and found that, among them, Myc-6 significantly suppresses malignant phenotypes of human osteosarcoma MG63 cells both in vitro and in vivo. Intriguingly, knockdown of the putative Myc-6 target MALAT1 encoding long noncoding RNA remarkably impaired cell growth of MG63 cells. Collectively, our present findings strongly suggest that Myc-6 exerts its tumor-suppressive ability at least in part through the specific down-regulation of MALAT1 .
- Subjects
OSTEOSARCOMA; IMIDAZOLES; POLYAMIDES; PYRROLES; GENE amplification; TRANSCRIPTION factors
- Publication
FEBS Open Bio, 2014, Vol 4, p328
- ISSN
2211-5463
- Publication type
Article
- DOI
10.1016/j.fob.2014.03.004