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- Title
Percutaneous prick test irritant threshold concentrations for eight allergens in healthy nonsedated dogs in the USA.
- Authors
Carnett, Matthew J. H.; Plant, Jon D.
- Abstract
Background: Percutaneous testing (PT) is preferred to intradermal testing in humans for the <italic>in vivo</italic> identification of allergen hypersensitivity, but the methodology has not been well described for use in dogs with atopic dermatitis. Hypothesis/Objectives: To identify the irritant threshold concentrations (ITC) of eight aeroallergens using a commercial prick test device in normal dogs. Animals: Twenty healthy, privately owned dogs. Methods: Percutaneous testing was performed using the GREER® Pick® System (Stallergenes Greer; Lenoir, NC, USA). Five dilutions of glycerinated extracts of <italic>Bromis inermis</italic>,<italic> Sorghum halepense</italic>,<italic> Chenopodium album</italic>,<italic> Ambrosia psilostachya</italic>,<italic> Salix nigra</italic> and <italic>Acer negundo</italic>, as well as four dilutions of <italic>Dermatophagoides farinae</italic> and <italic>D. pteronyssinus</italic> were included. Glycerinated histamine (6 mg/ml) and glycerin/Coca's solution were used for the positive and negative controls, respectively. Orthogonal wheal diameters were measured for each test site every 5 min for 25 min. Reactions were considered significant when the average wheal diameter was equal to or greater than the mean of the positive and negative controls. Results: Significant reactions were noted in five of 20 (25%) of dogs. The ITC (≤10% of dogs reacting) were 1:20 w/v for <italic>B. inermis</italic> and <italic>S. nigra</italic>, 1:400 w/v for <italic>D. farinae</italic> and 1:200 w/v for <italic>D. pteronyssinus</italic>. Conclusions and clinical importance: These results suggest that the pollen allergens evaluated in this study can be used for PT at their undiluted concentration (1:20 w/v) with a reasonable assurance of few false positive reactions in dogs. Dust mites require dilution for testing at the ITC.
- Subjects
DIAGNOSIS of dog diseases; DOG diseases; VETERINARY therapeutics; ALLERGENS; DOMESTICATION of dogs; VETERINARY dermatology; RANDOMIZED controlled trials
- Publication
Veterinary Dermatology, 2018, Vol 29, Issue 2, p117
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/vde.12514