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- Title
Exploring the Impact of Preoperative Sleep Fragmentation on Cognitive Function in Mice: The Role of Microglial Activation and Iron Metabolism.
- Authors
Chen, Yong; Yao, Peng; You, Yujuan; Xing, Xianliang; Wang, Xifeng; Zhou, Weijian; Hu, Yanhui; Lei, Enjun
- Abstract
Background. Perioperative neurocognitive disorders (PND) are a significant concern, particularly for aged individuals. Sleep fragmentation (SF), a common condition in older adults, is considered a risk factor for PND. The present study explored the impact of SF on cognitive function and its association with microglial activation and iron metabolism. Methods. Adult and aged C57BL/6J mice were subjected to tibial fracture surgery (TFS) and varying durations of SF. Cognitive function was assessed using the Morris water maze and fear conditioning experiments. Microglial activation was evaluated by measuring CD68 protein expression and inflammatory cytokine levels. Iron metabolism and ferroptosis-related proteins were also examined. Results. SF significantly impacted spatial memory and conditioned fear responses in mice, with aged mice showing greater susceptibility. Microglial activation, indicated by changes in CD68 protein expression and inflammatory cytokine levels, was observed in mice exposed to SF. Alterations in iron metabolism, as evidenced by changes in hippocampal iron content and expression of ferroptosis-related proteins, were also observed in these mice. Conclusion. SF can lead to significant cognitive impairment, particularly in aged mice, likely mediated through microglial activation and dysregulated iron metabolism. These findings provide novel insights into the pathogenesis of PND and suggest potential targets for intervention. Significance. This study illuminates the complex interactions between SF, microglial activation, and cognitive function. It highlights the importance of sleep quality for cognitive health in older adults and points to potential therapeutic strategies for preventing PND, including targeting microglial activation and iron metabolism.
- Subjects
IRON metabolism; COGNITION disorder risk factors; RISK assessment; PROTEINS; ELDER care; RESEARCH funding; NEUROGLIA; TIBIAL fractures; MACROSIALIN; PREOPERATIVE care; SOCIAL role; SLEEP duration; MICE; GENE expression; SLEEP deprivation; ANIMAL experimentation; RESEARCH; CELL death; COGNITION disorders; CYTOKINES; HIPPOCAMPUS (Brain); FACTOR analysis; SLEEP quality
- Publication
Journal of Clinical Pharmacy & Therapeutics, 2024, Vol 2024, p1
- ISSN
0269-4727
- Publication type
Article
- DOI
10.1155/2024/4376698