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- Title
Antidiarrheal potential of Distemonanthus benthamianus Baillon. extracts via inhibiting voltage-dependent calcium channels and cholinergic receptors.
- Authors
Nana, William Yousseu; Ateufack, Gilbert; Mbiantcha, Marius; Khan, Shamim; Rasheed, Hafiz Majid; Atsamo, Albert; Shah, Abdul Jabbar; Kamanyi, Albert; Khan, Taous
- Abstract
Objective: To evaluate spasmolytic mechanisms of aqueous and methanolic extracts from Distemonanthus benthamianus trunk-bark. Methods: Spasmolytic activities of extracts were evaluated in vitro on spontaneous and potassium chloride-induced jejunum contractions, or against cholinergic [acetylcholine (0.3 µmol/L)] stimulations. High performance liquid chromatography analysis of both extracts was performed in reference to standard compounds. Results: Extracts developed concentration-dependent inhibitory activities. The methanolic extract, which revealed better activity, produced spasmolytic and myorelaxant effects at concentrations of 0.01-0.30 mg/mL with EC50 of 0.06 and 0.09 mg/mL (95% CI: 0.03-0.3 mg/mL), respectively. Its anticholinergic effect was obtained at the same concentrations with EC50 of 0.11 mg/mL (95% CI: 0.03-0.3 mg/mL). Chromatograms showed the presence of gallic acid in both extracts, rutin being only detected in the aqueous extract. Conclusions: Distemonanthus benthamianus extracts exhibit verapamil and atropine-like activities, thus highlighting calcium channels and muscarinic receptors blocking potentials, which may be conveyed by some phenolic compounds. These results confirm the antidiarrheal activity of Distemonanthus benthamianus extracts.
- Subjects
CALCIUM channels; CHOLINERGIC receptors; ANTISPASMODICS; HIGH performance liquid chromatography; MUSCARINIC receptors; GALLIC acid
- Publication
Asian Pacific Journal of Tropical Biomedicine, 2019, Vol 9, Issue 11, p449
- ISSN
2221-1691
- Publication type
Article
- DOI
10.4103/2221-1691.270977