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- Title
Effects of Ectopic Expression of Rho\Rock Signaling Cascade Elements on PPARγ Expression.
- Authors
Kajabadi, E.; Ghoochani, A.; Ghaedi, K.; Peymani, M.; Nasr-Esfahani, M. H.; Baharvand, H.
- Abstract
Objective: Human embryonic stemcells, unlike mouse embryonic stem cells, are vulnerable to cell death upon dissociation. This apoptosis is dueto the activation of Rho/Rhock signaling pathway, which results in cytoskeletal phosphorylation due to myosin activation. E-cadherin is one of cytoskeleton elements that will be decreased after Rho/Rhock pathway activation. In this study we evaluate the effects of hESCs dissociation and so Rho/Rhock pathway activation on PPARγ expression in comparison with vital genes involved in colony formation like E-cadherin. Materials and Methods: Expression level analysis of PPARγ after dissociatinghESCs was performed by Real times PCR. To assess whether Rho/Rhock pathway effects on PPARγ is direct or not, co-transfection of two elements of thispathway (RhoA and PIP5K)withPPARγ was performed in CHO cell line and PPARγ expression wasanalyzed in RNA and protein levels by Real times PCR and western blotting. Results: PPARγ expression was reduced in dissociated hESCs the same as E-cadherin. Rho\Rock signaling cascade elements have direct effect on reducing of PPARγ expression. Conclusion: Our results showed that PPARγ, the same as vital genes involving in cytoskeletal structures, is directly vulnerable to Rho/Rock signaling pathway activation, which is due to dissociating hESCs
- Subjects
EMBRYONIC stem cells; PHOSPHORYLATION; MYOSIN
- Publication
Cell Journal (Yakhteh), 2013, Vol 15, Issue Sup 1, p51
- ISSN
2228-5806
- Publication type
Abstract