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- Title
In vivo imaging of lymph node migration of MNP- and (111)In-labeled dendritic cells in a transgenic mouse model of breast cancer (MMTV-Ras).
- Authors
Martelli C; Borelli M; Ottobrini L; Rainone V; Degrassi A; Russo M; Gianelli U; Bosari S; Fiorini C; Trabattoni D; Clerici M; Lucignani G; Martelli, Cristina; Borelli, Manuela; Ottobrini, Luisa; Rainone, Veronica; Degrassi, Anna; Russo, Micaela; Gianelli, Umberto; Bosari, Silvano
- Abstract
<bold>Purpose: </bold>The authors present a protocol for the in vivo evaluation, using different imaging techniques, of lymph node (LN) homing of tumor-specific dendritic cells (DCs) in a murine breast cancer model.<bold>Procedures: </bold>Bone marrow DCs were labeled with paramagnetic nanoparticles (MNPs) or (111)In-oxine. Antigen loading was performed using tumor lysate. Mature DCs were injected into the footpads of transgenic tumor-bearing mice (MMTV-Ras) and DC migration was tracked by magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT). Ex vivo analyses were performed to validate the imaging data.<bold>Results: </bold>DC labeling, both with MNPs and with (111)In-oxine, did not affect DC phenotype or functionality. MRI and SPECT allowed the detection of iron and (111)In in both axillary and popliteal LNs. Immunohistochemistry and γ-counting revealed the presence of DCs in LNs.<bold>Conclusions: </bold>MRI and SPECT imaging, by allowing in vivo dynamic monitoring of DC migration, could further the development and optimization of efficient anti-cancer vaccines.
- Publication
Molecular Imaging & Biology, 2012, Vol 14, Issue 2, p183
- ISSN
1536-1632
- Publication type
journal article
- DOI
10.1007/s11307-011-0496-0