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- Title
ANALIZA UNOR SUPLIMENTE ALIMENTARE ŞI MEDICAMENTE PENTRU DISFUNCŢIE ERECTILĂ PRIN METODA HPLC-UV.
- Authors
TERO-VESCAN, AMELIA; VARI, CAMIL-EUGEN; FILIP, CRISTINA; ŐSZ, BIANCA-EUGENIA; CEANĂ, DANIELA; IMRE, SILVIA; HANCU, GABRIEL
- Abstract
The popularity of dietary supplements and drugs for erectile dysfunction on the internet, permissive legal framework in this domain and purchasing these drugs from pharmacy on prescription only, explain why they are often subject to counterfeit. A HPLC-UV method for the identification and quantification of sildenafil, tadalafil and vardenafil in dietary supplements and drugs is described. Separation of these substances was performed on a Phenomenex C8 5μm 150x4.6 mm column, using as mobile phase a mixture of phase A = 0.1% formic acid in water and phase B = 0.1% formic acid in acetonitrile, in a concentration gradient, phase A concentration decreasing from 80 to 20% in 15 minutes. The detection was set at 262 nm. The supplements purchased from pharmacies did not contain phosphodiesterase 5 inhibitors, but in two supplements purchased on the internet, sildenafil was quantified (13.87 ± 1.79 mg/capsule and 18.34 ± 2.16 mg/capsule). The method was used for the determination of sildenafil in Viagra® 100 mg tablets purchased online, their content was higher by 35.80% from the declared composition.
- Publication
Acta Medica Transilvanica, 2014, Vol 19, Issue 4, p142
- ISSN
1453-1968
- Publication type
Article