We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Detection of high-risk human papillomavirus subtypes by RNA in situ hybridization in formalin-fixed paraffin-embedded tissue.
- Authors
Turashvili, Gulisa; Blay, Sasha; Conner, James; Dickson, Brendan; Demicco, Elizabeth; MacMillan, Christina
- Abstract
Objective: High-risk subtypes of human papillomavirus (HPV) cause approximately 5% of all carcinomas, including >95% of cervical cancers, 95% of anal cancers, and 70% of oropharyngeal cancers. Given that HPV status is the most powerful prognostic indicator, and HPV status may alter clinical management, accurate HPV detection within these tumours is crucial for optimal diagnosis and treatment. The objective of this study was to evaluate the performance of HPV ribonucleic acid in situ hybridization (RNA ISH) on selected diagnostically challenging cases. Methods: A total of 13 cases were identified with available data for p16 immunohistochemistry (IHC; all cases) and polymerase chain reaction (PCR; 7 cases). RNA ISH was performed manually on formalin-fixed paraffin-embedded (FFPE) tissue sections using a cocktail of horseradish peroxidase-labeled targeted probes to 18 high-risk HPV subtypes. Results: There were nine gynecological and four head and neck cases. HPV RNA ISH was concordant with PCR results in all cases. Of eleven p16-positive cases, only eight were positive for HPV by RNA ISH. Both cases with equivocal p16 expression were positive for HPV by RNA ISH. Three cases with diffuse p16 staining were negative for HPV by both RNA ISH and PCR. Conclusions: In this case series of diagnostically challenging tumours, HPV RNA ISH showed greater concordance with HPV PCR when compared with p16 IHC. RNA ISH has strong potential for utilization as a standalone clinical diagnostic test for detecting high-risk HPV subtypes in FFPE samples. Further validation studies with standardization and development of external quality control and proficiency programs are warranted.
- Subjects
HUMAN papillomavirus vaccines; IN situ hybridization; PHYSICIANS; PATHOLOGISTS; OROPHARYNGEAL cancer
- Publication
Canadian Journal of Pathology, 2021, Vol 13, Issue 1, p11
- ISSN
1918-915X
- Publication type
Article