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- Title
A new method to analyse the phenolic acids in peanut (Arachis hypogaea L.) rhizosphere soil.
- Authors
S. Li, Q.; Jia, X. L.; Ye, J. H.; Li, J. J.; Luo, X. M.; Lin, W. X.
- Abstract
A new method was developed to quantify the phenolic acids in soil. It is based on alkali (NaOH)-ethylacetate extraction (LLE) followed by gas chromatography-tandem mass spectrometry (GC-MS/MS). The Na2SO4 was used to improve the silanization (pre-column derivatization, to reduce the boiling point and improved molecular stability of phenolic compounds) effect and to improve the signal-to-noise ratio. Eleven phenolic acids were analyzed in peanut (Arachis hypogaea L.) cultured rhizosphere soil. The highest extraction recovery rates of the phenolic acids were achieved, when the alkaline soil extract was made acidic to pH of 2.5. Signal to noise ratio was improved (Higher the signal-to-noise ratio (SNR), the better detection effect) by 12.33 times. The matrix matched standard was used to avoid the soil matrix-effects and the coefficient R2 was > 0.99 for each phenolic acid. The sensitivity, accuracy and precision of analysis method were evaluated by limit of detection (LOD < 2.97 ng/g), standard recovery rate (90-105%) and relative standard deviation (RSD < 5.94%), respectively. The method was applied to soil samples and accurate contents of phenolic acids extracted form soil were calculated by the matrix matched calibrations and extraction recovery rates.
- Subjects
PHENOLIC acids; RHIZOSPHERE; ARACHIS; SODIC soils; SIGNAL-to-noise ratio
- Publication
Allelopathy Journal, 2019, Vol 48, Issue 2, p217
- ISSN
0971-4693
- Publication type
Article
- DOI
10.26651/allelo.j/2019-48-2-1256