We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Human Papillomavirus-Associated Oral Epithelial Dysplasia: A Practical Approach to Make the Diagnosis.
- Authors
Parchami, Kiarash; Derakhshan, Samira; Saffar, Hana; Aminishakib, Pouyan; Shamshiri, Ahmad Reza; Afshar, Samaneh
- Abstract
Background: High-risk Human Papillomavirus (HPV) genotypes are found in malignant oral epithelial lesions, and HPV infection is proposed as a risk factor for initiating Squamous cell carcinoma (SCC) in the head and neck region. This study suggests a practical approach to detect HPV in HPVassociated oral epithelial dysplasia (HAOED). Methods: Fifty-four oral epithelial dysplasia specimens were examined, comprising twenty-seven cases diagnosed with highgrade dysplasia and twenty-seven cases diagnosed with lowgrade dysplasia using a binary grading system. To assess the cases for HPV, the specimens were examined for p16 protein using an immunohistochemical (IHC) study, and then, the Chromatin In Situ Hybridization (CISH) test was performed for all positive cases. Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) was performed on CISH-positive specimens to assess the outcome. This cross-sectional study was conducted in 2020 at Tehran University of Medical Science. SPSS software version 22.0 was used to perform the Chi square or Fisher's exact test to examine the relationship between variables (statistically significant level P<0.05). Results: The expression of p16 protein was not associated with the severity of epithelial dysplasia (81.5% in low-grade and 59.2% in high-grade cases) (P=0.16). Moreover, according to the CISH test result, 9.25% of all specimens were positive (P>0.99), and in the nine cases, undergone the ChIP-PCR study, two cases (22.2%) showed positivity for HPV-16, while one case (11.1%) demonstrated positivity for HPV-51. Conclusion: Regarding HAOED, here, we proposed a step-bystep combination approach using different diagnostic methods, including IHC for p16 protein, CISH, and ChIP-PCR based on a complementary algorithm.
- Subjects
IRAN; ORAL microbiology; PAPILLOMAVIRUS disease diagnosis; PROTEIN analysis; SQUAMOUS cell carcinoma; CROSS-sectional method; MOUTH tumors; IN situ hybridization; RESEARCH funding; HEAD &; neck cancer; POLYMERASE chain reaction; FISHER exact test; CHI-squared test; SEVERITY of illness index; EPITHELIUM; IMMUNOHISTOCHEMISTRY; GENE expression; DATA analysis software; DISEASE risk factors
- Publication
Iranian Journal of Medical Sciences, 2024, Vol 49, Issue 3, p186
- ISSN
0253-0716
- Publication type
Article
- DOI
10.30476/IJMS.2023.96202.2897