We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Genetic and epigenetic regulation of major histocompatibility complex class I gene expression in bovine trophoblast cells.
- Authors
Shi, Bi; Thomas, Aaron J.; Benninghoff, Abby D.; Sessions, Benjamin R.; Meng, Qinggang; Parasar, Parveen; Rutigliano, Heloisa M.; White, Kenneth L.; Davies, Christopher J.
- Abstract
Problem The regulatory mechanisms governing differential expression of classical major histocompatibility complex ( MHC) class I ( MHC-Ia) and non-classical MHC class I ( MHC-Ib) genes are poorly understood. Method of study Quantitative reverse transcription- polymerase chain reaction ( PCR) was used to compare the abundance of MHC-I transcripts and related transcription factors in peripheral blood mononuclear cells ( PBMC) and placental trophoblast cells ( PTC). Methylation of MHC-I CpG islands was detected by bisulfite treatment and next-generation sequencing. Demethylation of PBMC and PTC with 5′-aza-deoxycytidine was used to assess the role of methylation in gene regulation. Results MHC-I expression was higher in PBMC than PTC and was correlated with expression of IRF1, class II MHC transactivator ( CIITA), and STAT1. The MHC-Ia genes and Bo LA- NC1 were devoid of CpG methylation in PBMC and PTC. In contrast, CpG sites in the gene body of Bo LA- NC2, - NC3, and - NC4 were highly methylated in PBMC but largely unmethylated in normal PTC and moderately methylated in somatic cell nuclear transfer PTC. In PBMC, demethylation resulted in upregulation of MHC-Ib by 2.8- to 6-fold, whereas MHC-Ia transcripts were elevated less than 2-fold. Conclusion DNA methylation regulates bovine MHC-Ib expression and is likely responsible for the different relative levels of MHC-Ib to MHC-Ia transcripts in PBMC and PTC.
- Subjects
MAJOR histocompatibility complex genetics; TROPHOBLAST; REVERSE transcriptase polymerase chain reaction; MONONUCLEAR leukocytes; DEOXYCYTIDINE; DNA methylation
- Publication
American Journal of Reproductive Immunology, 2018, Vol 79, Issue 1, pn/a
- ISSN
1046-7408
- Publication type
Article
- DOI
10.1111/aji.12779