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- Title
Development of an electrochemical-surface plasmon dual biosensor based on carboxylated conducting polymer thin films.
- Authors
Sriwichai, Saengrawee; Janmanee, Rapiphun; Phanichphant, Sukon; Shinbo, Kazunari; Kato, Keizo; Kaneko, Futao; Yamamoto, Tadashi; Baba, Akira
- Abstract
ABSTRACT A biosensing platform based on the covalent attachment of biomolecules on electropolymerized carboxylated conducting polymers, poly(3-aminobenzoic acid) and poly(3-pyrrole carboxylic acid), were developed for the selective simultaneous detection of two biomolecules using electrochemical-surface plasmon resonance (EC-SPR) spectroscopy. The surface morphology of the developed biosensors was studied by scanning electron microscopy and atomic force microscopy. The EC-SPR dual biosensor was developed for the label-free, simultaneous, and selective detection of glucose and human immunoglobulin G (IgG). A change in current density was clearly observed after the injection of glucose, whereas a change in SPR reflectivity was clearly observed after the injection of human IgG. The present work demonstrates the potential of this biosensing platform for real sample analysis in the future. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45641.
- Subjects
BIOSENSORS; POLYMER films; BIOMOLECULES; SURFACE plasmon resonance; SCANNING electron microscopy
- Publication
Journal of Applied Polymer Science, 2018, Vol 135, Issue 1, pn/a
- ISSN
0021-8995
- Publication type
Article
- DOI
10.1002/app.45641