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- Title
IN VITRO PROPAGATION OF SEVERAL VACCINIUM CORYMBOSUM L. AND VACCINIUM VITIS-IDAEA L. CULTIVARS.
- Authors
Ostrolucká, Mária Gabriela; Gajdošová, Alena; Ondrušková, Emilia; Libiaková, Gabriela
- Abstract
In the paper the efficient in vitro regeneration and propagation systems in several Vaccinium corymbosum L. and Vaccinium vitis-idaea L. cultivars are described. It was found that shoot regeneration ability is highly dependent on the cultivar and cytokinin applied. Zeatin showed itself to be efficient for axillary and adventitious shoot induction in both species. A zeatin concentration of 2 mg l-1 was the best for axillary shoot regeneration in V. corymbosum, while zeatin in a concentration of 0.75 - 1.5 mg l-1 was suitable for V. vitis-idaea cultivars. Multiple adventitious shoot formation on leaf explants of V. corymbosum occurred on the medium with 0.5 mg l-1 zeatin. In V. vitis-idaea indirect shoot regeneration was observed in cv. 'Red Pearl' after transfer of the calli on the medium with 0.5 mg l-1 zeatin. For long-term proliferation of in vitro regenerated axillary or adventitious shoots an AN medium with 0.5 mg l-1 zeatin was successfully used. Satisfactory rooting of isolated microshoots was achieved in vitro on an AN medium supplemented with 0.8 mg l-1 IBA and 0.8 g l-1 charcoal.
- Subjects
VACCINIUM vitis-idaea; CULTIVARS; VEGETATIVE propagation; CYTOKININS; PLANT shoots
- Publication
Latvian Journal of Agronomy / Agronomija Vestis, 2009, Issue 12, p75
- ISSN
1691-3485
- Publication type
Article