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- Title
BPH1, a novel substrate receptor of CRL3, plays a repressive role in ABA signal transduction.
- Authors
Woo, Og-Geum; Kim, Soon-Hee; Cho, Seok Keun; Kim, Sang-Hoon; Lee, Han Nim; Chung, Taijoon; Yang, Seong Wook; Lee, Jae-Hoon
- Abstract
Key message: BPH1 acts as a substrate receptor of CRL3 complex and negatively regulates ABA-mediated cellular responses. The study on its function provides information that helps further understand the relationship between ABA signaling and UPS.Abstract: Abscisic acid (ABA) plays a crucial role in a variety of cellular processes, including seed dormancy, inhibition of seedling growth, and drought resistance in plants. Cullin3-RING E3 ligase (CRL3) complex is a type of multi-subunit E3 ligase, and BTB/POZ protein, a component of CRL3 complex, functions as a receptor to determine a specific substrate. To elucidate the CRL3 complex that participates in ABA-mediated cellular processes, we first investigated ABA-inducible <italic>BTB/POZ</italic> genes based on data from the AtGenExpress Visualization Tool (AVT). We then isolated an ABA-inducible gene encoding a potential CRL3 substrate receptor in <italic>Arabidopsis, BPH1</italic> (<italic>BTB/POZ protein hypersensitive to ABA 1</italic>). The isolate gene has a BTB/POZ domain and a NPH3 domain within its N-terminal and C-terminal region, respectively. Yeast two-hybrid and co-immunoprecipitation assays showed that BPH1 physically interacted with cullin3a, a scaffold protein of CRL3, suggesting that it functions as an <italic>Arabidopsis</italic> CRL3 substrate receptor. The functional mutation of <italic>BPH1</italic> caused delayed seed germination in response to ABA and enhanced sensitivity by NaCl and mannitol treatments as ABA-related stresses. Moreover, <italic>bph1</italic> mutants exhibited enhanced stomatal closure under ABA application and reduced water loss when compared with wild-type, implying their enhanced tolerance to drought stress. Based on the information from microarray/AVT data and expression analysis of various ABA-inducible genes between wild-type and <italic>bph1</italic> plants following ABA treatments, we concluded loss of BPH1 resulted in hyper-induction of a large portion of ABA-inducible genes in response to ABA. Taken together, these results show that BPH1 is negatively involved in ABA-mediated cellular events.
- Subjects
ARABIDOPSIS; ABSCISIC acid; BENIGN prostatic hyperplasia; DROUGHT tolerance; IMMUNOPRECIPITATION
- Publication
Plant Molecular Biology, 2018, Vol 96, Issue 6, p593
- ISSN
0167-4412
- Publication type
Article
- DOI
10.1007/s11103-018-0717-x