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- Title
The effects of adenosine receptor agonists and antagonists on cytochrome P450 extinction in the liver of rats in the course of acute necrotic pancreatitis.
- Authors
Celiński, Krzysztof; Mądro, Agnieszka; Czechowska, Grazyna; SŁomka, Maria; Prozorow-Król, Beata; Malm, Anna
- Abstract
Introduction: In the course of acute pancreatitis (AP) the liver is an organ especially exposed to damage. Aim of study: The aim of the experiments was to define a correlation between the extinction of cytochrome P450 in the liver of rats and adenosine receptor agonists and antagonists in the course of taurocholate - induced AP. Material and methods: The experiments were carried out on Wistar male rats weighing an average of 250 g. AP was induced by injecting 5% sodium taurocholate to the biliary-pancreatic duct. Prior to the induction of acute pancreatitis the animals were injected intraperitoneally with adenosine receptor selective agonists and antagonists: CGS 21680, ZM 241385, DPCPX, Phenylxantine, and IBMECA. The determination was performed in hepatic microsomes obtained by using Guengerich's method. Cytochrome P450 extinction was determined by Matsubar's technique. Results: The results revealed statistically significant decreasing of cytochrome P450 extinction after sodium taurocholate administration. Decreased level of extinction was also observed after combined administration of Phenylxantine+sodium taurocholate and ZM+sodium taurocholate. The level of cytochrom P450 extinction remained unchanged after administration of IB-MECA+sodium taurocholate in comparison to the control group. However, after DPCPX+sodium taurocholate and CGS+sodium taurocholate administration the extinction level of cytochrome P450 increased. Conclusions: The diverse influence of adenosine receptor agonists and antagonists used in the experiments on cytochrom P450 extinction seems to modify the course of the inflammatory process after using 5% sodium taurocholate.
- Subjects
PANCREATITIS; CYTOCHROME P-450; BILE ducts; PANCREATIC duct; ADENOSINES; INFLAMMATION
- Publication
Gastroenterologia Polska / Gastroenterology, 2008, Vol 15, Issue 5, p299
- ISSN
1232-9886
- Publication type
Article