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- Title
An Evaluation of Histological Techniques Used in Skeletochronological Age Estimation of Sea Turtles.
- Authors
Goshe, Lisa R.; Avens, Larisa; Bybee, Joanna; Hohn, Aleta A.
- Abstract
Skeletochronological analysis was used to compare stained and unstained cross sections of humeri from Kemp's ridley (Lepidochelys kempii) and loggerhead (Caretta caretta) sea turtles to determine if the 2 histological techniques yielded an equal number of visible lines of arrested growth (LAGs). Stained sections viewed at high magnification under a compound microscope revealed the presence of closely spaced and splitting LAGs, resulting in a greater number of individual LAG counts for these sections when compared to unstained and stained sections viewed at a lower magnification under a dissecting microscope. Prior studies have shown that some of these closely spaced LAGs are annual, and therefore the inability to detect such marks could result in a downward bias in age estimates.
- Subjects
SEA turtles; SKELETOCHRONOLOGY; LOGGERHEAD turtle; VETERINARY histology; LEPIDOCHELYS kempii; DISSECTING microscopes
- Publication
Chelonian Conservation & Biology, 2009, Vol 8, Issue 2, p217
- ISSN
1071-8443
- Publication type
Article
- DOI
10.2744/CCB-0777.1