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- Title
Endothelium-dependent vascular relaxation induced by Globularia alypum extract is mediated by EDHF in perfused rat mesenteric arterial bed.
- Authors
Chokri, Aziz; El Abida, Kaouakib; Zegzouti, Younes Filali; Ben Cheikh, Rachid
- Abstract
The vasodilatory effect of Globularia alypum L. (GA) extract was evaluated in rat mesenteric arterial bed pre-contracted by continuous infusion of phenylephrine (2-4 ng/mL). Bolus injections of GA elicited dose-response vasodilation, which was abolished after endothelium removal. Addition of a nitric oxide synthase inhibitor, NG-nitro- l-arginine methyl ester (100 µmol/L), alone or in the presence of a cyclooxygenase inhibitor, indomethacin (10 µmol/L), did not significantly affect the vasodilation of the mesenteric arterial bed in response to GA extract. These results suggest that GA-induced vasodilation is endothelium dependent but nitric oxide and prostacyclin independent. In the presence of high K+ (60 mmol/L), the GA vasodilatory effect was completely abolished, suggesting that the vasodilation effect is mediated by hyperpolarization of the vascular cells. Also, pre-treatment with atropine (a muscarinic receptors antagonist) antagonized the GA-induced vasodilation, suggesting that the vasodilatory effect is mainly mediated by the endothelium-derived hyperpolarizing factor through activation of endothelial muscarinic receptors.
- Subjects
ENDOTHELINS; RELAXATION for health; PLANTAGINACEAE; PLANT extracts; VASODILATION; MESENTERIC artery; ENDOTHELIUM-derived hyperpolarizing factor
- Publication
Canadian Journal of Physiology & Pharmacology, 2012, Vol 90, Issue 5, p607
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/y2012-035