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- Title
Co-Administration of Insulin-Like Growth Factor (IGF)-I and IGF-Binding Protein-1 Stimulates Wound Healing in Animal Models.
- Authors
Tsuboi, Ryoji; Chog-Ming Shi, Ryoji; Sato, Chiyo; Cox, George N.; Ogawa, Hideoki
- Abstract
The stimulatory effect of recombinant human insulin-like growth factor-I (rhIGF-I) and recombinant human insulin-like growth-factor-binding protein-1 (rhIGFBP-1) on wound healing was assessed using diabetic db/db mice and normal rabbits. Full-thickness wounds of 6 mm diameter were prepared on the backs of diabetic C57BL/KsJ <em>db/db</em> mice and on the inner sides of normal rabbit ears. Various concentrations of rhIGF-I and/or rhIGFBP-1 were applied locally to the open wounds of <em>db/db</em> mice once daily for 5 d and to the covered wounds of normal rabbits once after wounding. Sections of the wounds were evaluated histologically on the seventh or eighth day by measuring re-epithelialization (%), area of granulation tissue (mm2), and capillary numbers. Wound repair was accelerated by each of the treatments in descending order of rhIGF-I plus rhIGFBP-1, rhIGF-I, rhIGFBP-1, and vehicle alone. In <em>db/db</em> mice, the combination of 50 μg rhIGF-I and 165 μg rhIGFBP-1 (equimolar ratio) significantly stimulated granulation tissue formation (p < 0.01) and capillary numbers (p < 0.05). Doses of rhIGFBP-1 greater than 16.5 μg were required for significant acceleration of the healing stimulated by 50 μg of rhIGF-I. In normal rabbits, co-administration of 10 μg rhIGF-I and 33 μg rhIGFBP-1 (equimolar ratio) significantly stimulated all three wound-healing parameters (p < 0.01), with such stimulation being much greater than that induced by rhIGF-I alone. Interestingly, rhIGFBP-1 alone showed a mild stimulatory activity on wound healing in both models despite its lack of mitogenic activity <em>in vitro.</em> These results demonstrate that rhIGFBP-1 enhances the stimulatory activity of rhIGF-I on wound healing and suggest the clinical utility of the co-administration of rhIGF-I and rhIGFBP-1 for wound repair.
- Subjects
WOUND healing; ANIMALS; GROWTH factors; CYTOKINES; INSULIN; PROTEINS
- Publication
Journal of Investigative Dermatology, 1995, Vol 104, Issue 2, p199
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12612755