We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Ultrastructural Changes in Red Blood Cells Following Pulsed Irradiation In Vitro.
- Authors
Tan, Oon Tian; Morelli, Joseph G.; Whitaker, Diana; Boll, James; Murphy, George
- Abstract
The careful choice of a combination of laser parameters such as wavelength, pulse duration, and dose has provided a means for confining laser energy to specific targets within tissue such as oxyhemoglobin within the cutaneous microvasculature. In the process of achieving such vascular selectivity, certain ultrastructural changes in red blood cell (RBC) cytoplasm have been observed, such as the generation of intracy-toplasmic electron-lucent spherical structures. These structures, ranging in size from 80 to 1000Å, were seen in RBCs exposed to laser doses at and above threshold, and appeared to represent a morphologically novel form of highly-specific tissue injury. This in vitro study using RBC in phosphate buffered saline (PBS) was undertaken to better understand the mechanism(s) that could have been responsible for these unique morphologic changes. We conclude that the intracy-toplasmic electron-lucent spherical structures seen within RBCs were heat-fixed molds formed around vaporized water bubbles and were not produced by the release of oxygen from the oxyhemoglobin moiety during 577-nm laser irradiation.
- Subjects
ERYTHROCYTES; IRRADIATION; OXYHEMOGLOBIN; HEMOGLOBINS; MICROCIRCULATION disorders; CYTOPLASM
- Publication
Journal of Investigative Dermatology, 1989, Vol 92, Issue 1, p100
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep13071307