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- Title
Characterization of hepatitis B virus (HBV) preS/S gene mutations in blood donors with occult HBV infection in the Baoji area of North China.
- Authors
Liao, Hao; Liu, Yan; Chen, Jianhong; Ding, Weiping; Li, Xiaodong; Xu, Zhihui; Yang, Yuan; Chen, Rongjuan; Si, Lanlan; Xu, Xiaoyuan; Guo, Jianxun; Xu, Dongping
- Abstract
<bold>Background: </bold>Occult hepatitis B virus (HBV) infection (OBI) in blood donors was investigated in the Baoji area of North China, and OBI-related viral mutations in donors were characterized.<bold>Study Design and Methods: </bold>In total, 110,843 blood donor samples that were consecutively collected from December 2011 to March 2015 at the Baoji Blood Center were examined. Hepatitis B surface antigen-negative and HBV DNA-positive OBI samples were amplified for sequence analysis of OBI-related mutations in the HBV preS/S region. HBV genomes from 108 adult patients with chronic hepatitis B from North China were used as controls.<bold>Results: </bold>OBI was detected in 60 (1:1847) individual blood donors. All OBI samples were negative for hepatitis B e-antigen, and 55 were positive for anti-hepatitis B core antigen. The preS/S genes were successfully sequenced for 43 OBI samples. OBI-related S gene mutations in the major hydrophilic region were detected more frequently in blood donors with OBI than that in controls (51.16 vs. 12.96%; p < 0.01). Specifically, the incidence of five OBI-related major hydrophilic region mutations (sS117T, sT118K, sT131N, sT134Y/L, and sD144E) was significantly higher in blood donors with OBI than in controls. In addition, the coexistence of multiple OBI-related mutations in the major hydrophilic region was detected more frequently in donors than in controls (30.23 vs. 1.85%; p < 0.01), and preS deletions greater than 33 base pairs also were detected more frequently in blood donors with OBI than in controls.<bold>Conclusion: </bold>OBI in blood donors should be addressed attentively in the Baoji area of North China, and HBV preS/S gene mutations may play an important role in OBI prevalence in the area.
- Subjects
CHINA; HEPATITIS B virus; GENETIC mutation; DONOR blood supply; BLOOD donors; DISEASE prevalence; PUBLIC health; HEPATITIS B; HEPATITIS viruses; PROTEIN precursors; VIRAL antigens
- Publication
Transfusion, 2017, Vol 57, Issue 3pt2, p857
- ISSN
0041-1132
- Publication type
journal article
- DOI
10.1111/trf.14046