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- Title
Stimulation of Interleukin 6-Like B-Cell Differentiation Factor Production in Human Adherent Synovial Cells by Recombinant Interleukin 1.
- Authors
Harigai, M.; Hara, M.; Norioka, K.; Kitani, A.; Hirose, T.; Suzuki, K.; Kawakami, M.; Masuda, K.; Shinmei, M.; Kawagoe, M.; Nakamura, H.
- Abstract
Abnormal production of immunoglobulin in the joint space is frequently observed in patients with rhecumatoid arthritis (RA). We have previously demonstrated that adherent synovial cells (ASC) from patients with RA are involved in B-cell differentiation by their spontaneous production of B-cell differentiation factor (BCDF). The regulation of the production of this factor, however, has not yet been described. We investigated the effects of recombinant interleukin aα and β (eIL-1α and rIL-1β) on the production of BCDF in ASC increased production of BCDF was observed with increased rIL-1 concentration. Production of BCDF was detected 3 h after exposure of ASC to rIL-1 and increased throughout a 48-h culture. This BCDF assayed on SKW6-WL4 cells, was found to share a common active site with interleukin 6. The effect of rIL-1 was almost neutralized by anti-IL-1 antibody and the addition of polymiyxin B did not diminish the effect of rIL-1, indicating the rIL-1 itself stimulates ASC in vitro. These results suggest that IL-1 may play a regulatory role in the production of BCDF in synovial tissue.
- Subjects
INTERLEUKIN-6; INTERLEUKINS; IMMUNOGLOBULINS; GLOBULINS; BLOOD proteins; CELL differentiation
- Publication
Scandinavian Journal of Immunology, 1989, Vol 29, Issue 3, p289
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1989.tb01127.x