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- Title
Inhibitor of Interleukin 1 in Normal Human Urine.
- Authors
Svenson, M.; Bendtzen, K.
- Abstract
We investigated whether urine from normal individuals contains inhibitors of interleukin 1 (IL- 1). Diafiltered urine from normal afebrile donors suppressed IL-1-induced interleukin 2 (IL-2) activity of mouse thymocyte supernatants. These supernatants, however, strongly suppressed IL-2-induced [3H]thymidine incorporation into the IL-2-sensitive cell line CTLL-2, whereas the urine preparations did not. This phenomenon was caused by an increased amount of thymidine secreted by the urine-treated thymocytes. Therefore, in order lo prevent interference, experiments were carried out with excess ['H]thymidine. Under these circumstances, suppression of IL-1- and to a lesser extent IL-2-induced DNA synthesis was still observed, whereas the synergistic effect of IL-I on lL-2-induced DNA synthesis was only marginally reduced. We conclude that suppression of IL-1-induced IL-2 production by mouse thymocytes is a major effect of the IL-I-inhibitory factor(s)in normal urine. When the murine EL 4 cell line was used, the diafiltered urine failed lo inhibit IL-l-induced lL-2 production. The detection of an IL-1 inhibitor in urine is therefore dependent on the target cells as well as the effects of IL-1 on these cells.
- Subjects
URINE; INTERLEUKIN-1; THYMIDINE; CELL lines; THYMINE; DNA synthesis
- Publication
Scandinavian Journal of Immunology, 1988, Vol 27, Issue 5, p593
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1988.tb02387.x