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- Title
CENP-A confers a reduction in height on octameric nucleosomes.
- Authors
Miell, Matthew D D; Fuller, Colin J; Guse, Annika; Barysz, Helena M; Downes, Andrew; Owen-Hughes, Tom; Rappsilber, Juri; Straight, Aaron F; Allshire, Robin C
- Abstract
Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.
- Subjects
HUMAN chromatin; PROTEIN structure; DROSOPHILA; HISTONES; ATOMIC force microscopy
- Publication
Nature Structural & Molecular Biology, 2013, Vol 20, Issue 6, p763
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.2574