We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Harnessing Catalytic RNA Circuits for Construction of Artificial Signaling Pathways in Mammalian Cells.
- Authors
Wu, Chao‐Qun; Wu, Ruo‐Yue; Zhang, Qiu‐Long; Wang, Liang‐Liang; Wang, Yang; Dai, Chu; Zhang, Chen‐Xi; Xu, Liang
- Abstract
Engineering of genetic networks with artificial signaling pathways (ASPs) can reprogram cellular responses and phenotypes under different circumstances for a variety of diagnostic and therapeutic purposes. However, construction of ASPs between originally independent endogenous genes in mammalian cells is highly challenging. Here we report an amplifiable RNA circuit that can theoretically build regulatory connections between any endogenous genes in mammalian cells. We harness the system of catalytic hairpin assembly with combination of controllable CRISPR‐Cas9 function to transduce the signals from distinct messenger RNA expression of trigger genes into manipulation of target genes. Through introduction of these RNA‐based genetic circuits, mammalian cells are endowed with autonomous capabilities to sense the changes of RNA expression either induced by ligand stimuli or from various cell types and control the cellular responses and fates via apoptosis‐related ASPs. Our design provides a generalized platform for construction of ASPs inside the genetic networks of mammalian cells based on differentiated RNA expression.
- Subjects
GENE expression; CATALYTIC RNA; GENETIC engineering; CELLULAR signal transduction; HAIRPIN (Genetics); MESSENGER RNA; CRISPRS
- Publication
Angewandte Chemie, 2024, Vol 136, Issue 14, p1
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.202319309