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- Title
Genomic and proteomic characterization of vB_SauM-UFV_DC4, a novel Staphylococcus jumbo phage.
- Authors
da Silva, Jéssica Duarte; Melo, Luís D. R.; Santos, Sílvio B.; Kropinski, Andrew M.; Xisto, Mariana Fonseca; Dias, Roberto Sousa; da Silva Paes, Isabela; Vieira, Marcella Silva; Soares, José Júnior Ferreira; Porcellato, Davide; da Silva Duarte, Vinícius; de Paula, Sérgio Oliveira
- Abstract
Staphylococcusaureus is one of the most relevant mastitis pathogens in dairy cattle, and the acquisition of antimicrobial resistance genes presents a significant health issue in both veterinary and human fields. Among the different strategies to tackle S. aureus infection in livestock, bacteriophages have been thoroughly investigated in the last decades; however, few specimens of the so-called jumbo phages capable of infecting S. aureus have been described. Herein, we report the biological, genomic, and structural proteomic features of the jumbo phage vB_SauM-UFV_DC4 (DC4). DC4 exhibited a remarkable killing activity against S. aureus isolated from the veterinary environment and stability at alkaline conditions (pH 4 to 12). The complete genome of DC4 is 263,185 bp (GC content: 25%), encodes 263 predicted CDSs (80% without an assigned function), 1 tRNA (Phe-tRNA), multisubunit RNA polymerase, and an RNA-dependent DNA polymerase. Moreover, comparative analysis revealed that DC4 can be considered a new viral species belonging to a new genus DC4 and showed a similar set of lytic proteins and depolymerase activity with closely related jumbo phages. The characterization of a new S. aureus jumbo phage increases our understanding of the diversity of this group and provides insights into the biotechnological potential of these viruses. Key points: • vB_SauM-UFV_DC4 is a new viral species belonging to a new genus within the class Caudoviricetes. • vB_SauM-UFV_DC4 carries a set of RNA polymerase subunits and an RNA-directed DNA polymerase. • vB_SauM-UFV_DC4 and closely related jumbo phages showed a similar set of lytic proteins.
- Subjects
REVERSE transcriptase; TRANSFER RNA; RNA replicase; PROTEOMICS; BACTERIOPHAGES; RNA polymerases; STAPHYLOCOCCUS
- Publication
Applied Microbiology & Biotechnology, 2023, Vol 107, Issue 23, p7231
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-023-12743-6