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- Title
A bacteriophage mimic of the bacterial nucleoidassociated protein Fis.
- Authors
Chakraborti, Soumyananda; Balakrishnan, Dhanasekaran; Trotter, Alexander J.; Gittens, William H.; Yang, Ally W. H.; Jolma, Arttu; Paterson, Joy R.; S'wia?tek, Sylwia; Plewka, Jacek; Curtis, Fiona A.; Bowers, Laura Y.; Pålsson, Lars-Olof; Hughes, Timothy R.; Taube, Michał; Kozak, Maciej; Heddle, Jonathan G.; Sharples, Gary J.
- Abstract
We report the identification and characterization of a bacteriophage ?-encoded protein, NinH. Sequence homology suggests similarity between NinH and Fis, a bacterial nucleoidassociated protein (NAP) involved in numerous DNA topology manipulations, including chromosome condensation, transcriptional regulation and phage site-specific recombination. We find that NinH functions as a homodimer and is able to bind and bend doublestranded DNA in vitro. Furthermore, NinH shows a preference for a 15 bp signature sequence related to the degenerate consensus favored by Fis. Structural studies reinforced the proposed similarity to Fis and supported the identification of residues involved in DNA binding which were demonstrated experimentally. Overexpression of NinH proved toxic and this correlated with its capacity to associate with DNA. NinH is the first example of a phage-encoded Fis-like NAP that likely influences phage excision-integration reactions or bacterial gene expression.
- Subjects
BACTERIAL proteins; BACTERIOPHAGES; BACTERIAL genes; GENE expression; DNA
- Publication
Biochemical Journal, 2020, Vol 477, Issue 7, p1345
- ISSN
0264-6021
- Publication type
Article
- DOI
10.1042/BCJ20200146