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- Title
Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells with Synthetic Coral Matrix in the Presence or Absence of Growth Factors from the Platelet Rich-rich Plasma.
- Authors
Mahanani, Erlina Sih; Ana, Ika Dewi; Bachtiar, Indra; Yin Xiao
- Abstract
To solve problems in using natural sea corals with high biocompatibility, good osteoconductivity, and ideal degradability, a synthetic three-dimensional coral matrix was fabricated and observed for its capability to enhance osteogenic differentiation of human mesenchymal stem cells (h-MSCs), in the presence or absence of growth factors (GFs) supplied from platelet-rich plasma (PRP). Expressions of runx, osterix, and osteocalcin were investigated, following cell attachment, and proliferation analysis. Ectopic bone formation in nonosseous tissues of Sprague-Dawley rats at predetermined time intervals was investigated, including mineralized tissue growth in vivo. The 3D synthetic coral matrix (SCM) can interact with the GF cocktail in the PRP and MSCs to generate and secret bone extracellular matrix (ECM) both in vitro and in vivo. The matrix supplied with GF cocktail from the PRP provided an ideal microenvironment for MSCs to attach, proliferate, and differentiate into osteoblast faster, as indicated by the expression levels of runx2, osterix, and osteocalcin. The high capability of SCMs to enhance bone formation has been proven by the formation of ectopic bones in the nonosseous environment. The incorporated PRP provided blood proteins such as fibrin to slow down matrix degradation, whereas GF supplied by the PRP stimulated h-MSCs to attach and proliferate onto the matrix. Moreover, the GF supplied by the PRP enhanced osteogenic differentiation and mineralization, accelerating bone regeneration. Valorization phases are needed to apply the SCM for bone tissue engineering in clinics.
- Subjects
MESENCHYMAL stem cell differentiation; PLATELET-rich plasma; BONE regeneration; GROWTH factors; FIBRIN; BLOOD proteins; CORALS; BONE growth
- Publication
Trends in Sciences, 2024, Vol 21, Issue 2, p1
- ISSN
2774-0226
- Publication type
Article
- DOI
10.48048/tis.2024.7168