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- Title
TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells.
- Authors
Abdel-Latif, Ahmed; Zuba-Surma, Ewa K.; Case, Jamie; Tiwari, Sumit; Hunt, Greg; Ranjan, Smita; Vincent, Robert; Srour, Edward; Bolli, Roberto; Dawn, Buddhadeb
- Abstract
The optimal medium for cardiac differentiation of adult primitive cells remains to be established. We quantitatively compared the efficacy of IGF-1, dynorphin B, insulin, oxytocin, bFGF, and TGF-β1 in inducing cardiomyogenic differentiation. Adult mouse skeletal muscle-derived Sca1+/CD45-/c-kit-/Thy-1+ (SM+) and Sca1-/CD45-/c-kit-/Thy-1+ (SM-) cells were cultured in basic medium (BM; DMEM, FBS, IGF-1, dynorphin B) alone and BM supplemented with insulin, oxytocin, bFGF, or TGF-β1. Cardiac differentiation was evaluated by the expression of cardiac-specific markers at the mRNA (qRT-PCR) and protein (immunocytochemistry) levels. BM+TGF-β1 upregulated mRNA expression of Nkx2.5 and GATA-4 after 4 days and Myl2 after 9 days. After 30 days, BM+TGF-β1 induced the greatest extent of cardiac differentiation (by morphology and expression of cardiac markers) in SM- cells. We conclude that TGF-β1 enhances cardiomyogenic differentiation in skeletal muscle-derived adult primitive cells. This strategy may be utilized to induce cardiac differentiation as well as to examine the cardiomyogenic potential of adult tissue-derived stem/progenitor cells.
- Subjects
CELL differentiation; TRANSFORMING growth factors-beta; INSULIN; OXYTOCIN; DYNORPHINS
- Publication
Basic Research in Cardiology, 2008, Vol 103, Issue 6, p514
- ISSN
0300-8428
- Publication type
Article
- DOI
10.1007/s00395-008-0729-9