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- Title
Deciphering 2-O-sulfated heparin interactome using Escherichia coli proteome chip.
- Authors
Tseng, C. Y.; Chen, C. S.; Hung, L. Y.; Artdita, C. A.; Peng, T. C.; Lin, T. Y.; Hsiao, S. H.
- Abstract
Escherichia coli can induce severe mastitis in dairy cows. The 2-0 sulfate group of heparin is crucial for E. coli virulence. However, the interaction of 2-0 sulfated heparin (2-OSH) induced by E. coli is still unknown. Herein, the E. coli proteome chip was used to reveal the 2-OSH interactome (2-OSH hits), via the combined analysis of interactions between heparin, 2-O-desulfated sulfated heparins (2-ODSH), and E. coli proteins. Specifically, a total of 58 proteins that can bind to 2-OSH were defined (2-OSH hits). Gene Ontology showed that 2-OSH hits were enriched in bacterial capsule and bacterial toxic components (P < 0.05); KEGG analysis confirmed these 2-OSH hits were bound to negatively charged heparin sulfate. Using the cut-off > 3SD, a total of 11 proteins were revealed to have significant repetitive variable sequence A[AK]R[QR][RE][KG].{0,4}R[SE]AA.[QK][AE] (P < 0.0001). This study revealed the 2-OSH hits through the E. coli proteome chip. These proteins can be used to analyze the molecular mechanism of E. coli induced mastitis.
- Subjects
ESCHERICHIA coli; HEPARIN; PROTEOMICS; MASTITIS diagnosis; BACTERIAL capsules; GENE ontology
- Publication
Journal of the Chinese Society of Animal Science, 2021, Vol 50, p163
- ISSN
0253-9187
- Publication type
Article