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- Title
Direct isolation of Francisella spp. from environmental samples.
- Authors
Petersen, J. M.; Carlson, J.; Yockey, B.; Pillai, S.; Kuske, C.; Garbalena, G.; Pottumarthy, S.; Chalcraft, L.
- Abstract
Aims: To develop a selective medium for isolation of F. tularensis, F. novicida and F. philomiragia from environmental samples. Methods and Results: A selective media, cysteine heart agar with 9% chocolatized sheep blood, containing polymyxin B, amphotericin B, cyclohexamide, cefepime and vancomycin (CHAB-PACCV) was developed and evaluated for growth of Francisella spp. No differences were observed in recovered colony forming units (CFUs) for F. tularensis, F. novicida and F. philomiragia on CHAB-PACCV vs nonselective CHAB. Growth of non- Francisella species was inhibited on CHAB-PACCV. When environmental samples were cultured on CHAB and CHAB-PACCV, only CHAB-PACCV allowed isolation of Francisella spp. Three new Francisella strains were isolated directly from seawater and seaweed samples by culture on CHAB-PACCV. Conclusions: CHAB-PACCV can be used for direct isolation of Francisella spp from environmental samples. Significance and Impact of the Study: Francisella spp. show a close association with environmental sources. Future utilization of CHAB-PACCV for isolation of Francisella spp. directly from environmental samples should prove valuable for investigating outbreaks and human infections attributed to environmental exposure.
- Subjects
FRANCISELLA; ENVIRONMENTAL sampling; SEAWATER; MARINE algae; AMPHOTERICIN B; VANCOMYCIN; POLYMYXIN; VIRUS isolation
- Publication
Letters in Applied Microbiology, 2009, Vol 48, Issue 6, p663
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/j.1472-765X.2009.02589.x