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- Title
Drug resistance and pulsed-field gel electrophoresis patterns ofLactococcus garvieaeisolates from culturedSeriola(yellowtail, amberjack and kingfish) in Japan.
- Authors
Kawanishi, M.; Kojima, A.; Ishihara, K.; Esaki, H.; Kijima, M.; Takahashi, T.; Suzuki, S.; Tamura, Y.
- Abstract
m. kawanishi, a. kojima, k. ishihara, h. esaki, m. kijima, t. takahashi, s. suzuki and y. tamura. 2005.To investigate the existing antimicrobial susceptibility and genetic characteristics ofLactococcus garvieaeisolates from culturedSeriolain Japan.Minimum inhibitory concentrations (MICs) of 14 antimicrobial agents for 170 isolates were determined using the agar dilution method. Seventy-five isolates (44·1%) were simultaneously resistant to erythromycin (EM) (MIC ≥ 2 μg ml−1), lincomycin (LCM) (MIC ≥ 128 μg ml−1) and oxytetracycline (OTC) (MIC ≥4 μg ml−1). Resistance to EM was grouped as intermediate- and high-level resistant by MIC values. All resistant isolates possessedermBandtet(S) genes. The number of different bands between pulsed-field gel electrophoresis patterns of 25 isolates and two ATCC strains (isolated in 1974), determined using two enzymes (ApaI andSmaI), did not exceed 3.The present resistance pattern observed withermBandtet(S) is similar to that observed in previous reports. Moreover, the genetic characteristics ofL. garvieaeisolates from a wide area in Japan in 2002 and ATCC strains were closely related.This study suggests that EM-, LCM- and OTC-resistant isolates have been present for 15 years and thatL. garvieaestrains with same origin have spread amongSeriolaspp. in Japan since 1974.
- Subjects
SERIOLA; LACTOCOCCUS; DRUG resistance; ELECTROPHORESIS; ELECTROCHEMISTRY; STREPTOCOCCACEAE
- Publication
Letters in Applied Microbiology, 2005, Vol 40, Issue 5, p322
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/j.1472-765X.2005.01690.x