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- Title
Incidence of antibiotic-resistantKlebsiella pneumoniaeandEnterobacterspecies in freshwater wetlands.
- Authors
Halda-Alija, L.
- Abstract
l. halda-alija. 2004.The aim of this study was to assess the incidence of Enterobacteriaceae (potential human and animal pathogens) in wetlands.Enterobacteriaceae, selected from the sediments and rhizosphere of wetland plantJuncus effususL., were analysed using classical microbiological methods, API20E, API20NE, fatty acid analyses, and 16S rRNA sequencing. Assessed virulence factors include antibiotic resistance, presence of plasmids and capsules.Klebsiella pneumoniae,Enterobacter cloacaeandEnterobacter asburiae, known human pathogens, were identified.K. pneumoniae16S rRNA gene sequence showed the significant hit (E < 0·001) with the unculturable bacteria obtained from faeces of elderly individuals (accession number) when Genbank database was used.Ent. asburiae16S rRNA gene sequence showed the significant hit with (E < 0·001) with the unculturable bacteria obtained from the pig gastrointestinal tract (accession number). The rate of antibiotic resistance (<50 μg ml−1) was high for ampicillin and cephalosporins for the most strains (75·7%) yet low (>10 to 20 μg ml−1) for kanamycin, tetracycline and chloramphenicol for all strains tested. Capsules were detected in all investigated strains. PCR detected membrane protein but not chromosomally encodedβ-lactamase.The antibiotic resistance of tested strains and presence of capsules (protect micro-organisms from phagocytosis) suggest that wetland sediments and rhizosphere present a potential reservoirs for enteric human and animal pathogens.
- Subjects
KLEBSIELLA pneumoniae; ENTEROBACTERIACEAE; DRUG resistance in microorganisms; JUNCUS effusus; RHIZOSPHERE; MICROBIOLOGY
- Publication
Letters in Applied Microbiology, 2004, Vol 39, Issue 5, p445
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/j.1472-765X.2004.01612.x