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- Title
Knockdown circTRIM28 enhances tamoxifen sensitivity via the miR-409-3p/HMGA2 axis in breast cancer.
- Authors
Yang, Shiyong; Zou, Changwu; Li, Yuxin; Yang, Xianguo; Liu, Wei; Zhang, Guannan; Lu, Nina
- Abstract
Background: Tamoxifen (TAM) is a frequently-used treatment for breast cancer (BC). But the TAM resistance seriously affects the patient therapeutic effect. Previous research indicated that circular RNAs (circRNAs) might participate in the regulatory processes of BC. Here, we discovered the parts of circular RNA tripartite motif-containing 28 (circTRIM28) in BC. Methods: CircTRIM28, microRNA-409-3p (miR-409-3p), and high mobility group AT-hook 2 (HMGA2) levels were perceived by qRT-PCR and western blot. Moreover, the biological functions of the cells were examined. Furthermore, dual-luciferase report was employed to reconnoiter the targeted relationship between miR-409-3p and circTRIM28 or HMGA2. Results: CircTRIM28 and HMGA2 were augmented, and the miR-409-3p was repressed in BC. Silencing circTRIM28 enhanced tamoxifen sensitivity and cell apoptosis, whereas hampered cell development in BC cells. In mechanism, circTRIM28 could sponge miR-409-3p to increase HMGA2. In addition, silencing circTRIM28 impeded tumor growth. Conclusion: CircTRIM28 facilitated the BC via miR-409-3p/HMGA2. Highlights: 1. The expression of circTRIM28 was upregulated in BC. 2. Silencing circTRIM28 enhanced tamoxifen sensitivity and cell apoptosis, whereas curbed cell proliferation, cell migration and invasion in BC cells. 3. CircTRIM28 expedited BC by targeting miR-409-3p. 4. MiR-409-3p repressed BC by retarding HMGA2.
- Subjects
TAMOXIFEN; BREAST cancer; CIRCULAR RNA; CELL migration; TUMOR growth
- Publication
Reproductive Biology & Endocrinology, 2022, Vol 20, Issue 1, p1
- ISSN
1477-7827
- Publication type
Article
- DOI
10.1186/s12958-022-01011-3