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- Title
Cell cycle kinetic analysis with flow cytometry in pea root meristem synchronized with aphidicolin.
- Authors
Sgorbati, Sergio; Sparvoli, Elio; Levi, Marisa; Grazia Galli, Maria; Citterio, Sandra; Chiatante, Donato
- Abstract
Meristematic cells in pea root were synchronized after treatment with aphidicolin. Flow cytometric analysis of DNA content variations revealed a variety of information: ca 50% of the cells were synchronized in the first part of the S phase; kinetic studies of the S-phase traverse revealed an increase of the DNA synthesis rate from early to late S; some of the cells in the meristem were non-cycling, arrested in G1 (19%) and G2 (12%). The possibility of using such a synchronized system in cytogenetic and biomolecular studies is discussed.
- Subjects
PEA genetics; PLANT cell cycle; MERISTEMS; PLANT roots; DNA replication; FLOW cytometry
- Publication
Physiologia Plantarum, 1991, Vol 81, Issue 4, p507
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1991.tb05092.x