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- Title
An Easy-to-Use Histological Technique for Small Biological Samples of Senegalese Sole Larvae.
- Authors
Santana, Lígia M. B. M.; Damasceno, Évila Pinheiro; Loureiro, Susana; Soares, Amadeu M. V. M.; Pousão-Ferreira, Pedro; Abessa, Denis M. S.; Martins, Roberto; Pavlaki, Maria D.
- Abstract
Histological techniques are a traditional and essential tool for adequately comprehending cellular morphology and physiology in several scientific fields, including ecotoxicological studies. Although bioassays generally recommend using small test organisms, based on advantages such as low maintenance costs and the possibility of a higher number of replicates, the histological process of such organisms is still challenging due to the difficulty of handling them. The present study proposes an accessible protocol for performing the histology of a biological sample of 8 mm maximum length and using histopathological biomarkers in Senegal sole larvae for ecotoxicological tests. Eight-day old larvae of Solea senegalensis obtained from breeders' natural spawns in cultivation conditions were submitted to histological processing by an adjusted protocol comprising fixation, agarose array pre-embedding, dehydration, paraffin embedding, slides' preparation, and image analysis by light optical microscope coupled to a digital camera. Low-cost and easy-to-use alternatives were applied in the technique execution. The histological steps were detailed, and S. senegalensis morphological features were presented. This can be considered valuable background information for ecotoxicological studies where changes in cell morphology and physiology are expected.
- Subjects
SENEGAL; HISTOLOGICAL techniques; CELL morphology; CELL physiology; SOLEA senegalensis; LARVAE; FISH spawning
- Publication
Applied Sciences (2076-3417), 2023, Vol 13, Issue 4, p2346
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app13042346